2O5V
Recombination mediator RecF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-05 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.897, 87.419, 52.898 |
| Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.610 |
| R-factor | 0.16907 |
| Rwork | 0.167 |
| R-free | 0.21423 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.579 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.052 | 0.436 |
| Number of reflections | 41165 | |
| <I/σ(I)> | 12 | 1.2 |
| Completeness [%] | 92.4 | |
| Redundancy | 2.4 | 49.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.5M (NH4)2SO4 20% Sucrose, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.5M (NH4)2SO4 20% Sucrose, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
