2O5U
Crystal structure of the PA5185 protein from Pseudomonas Aeruginosa strain PAO1- orthorhombic form (C222).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 124.039, 148.157, 58.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.19225 |
| Rwork | 0.191 |
| R-free | 0.22296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2av9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.697 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.091 | 0.427 |
| Number of reflections | 41694 | |
| <I/σ(I)> | 17.2 | 1.9 |
| Completeness [%] | 98.4 | 87.9 |
| Redundancy | 4.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 20% PEG 3350, 0.1M Bis-Tris, 0.05M MES, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
