2O58
Horse heart met manganese myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.414, 28.654, 62.940 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 19.470 - 1.650 |
| R-factor | 0.18148 |
| Rwork | 0.179 |
| R-free | 0.22346 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.569 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2D) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.690 | 26.690 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.057 | 0.030 | 0.278 |
| Number of reflections | 16239 | ||
| <I/σ(I)> | 14.4 | 39.4 | 4 |
| Completeness [%] | 99.3 | 99.6 | 96.9 |
| Redundancy | 4.23 | 4.33 | 3.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | Ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
