2O55

Crystal Structure of a putative glycerophosphodiester phosphodiesterase from Galdieria sulphuraria

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97923, 0.96400
Spacegroup name	P 31 2 1
Unit cell lengths	102.427, 102.427, 51.709
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.387 - 2.806
R-factor	0.195
Rwork	0.192
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.486
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.387	36.387	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.099	0.076	0.197
Number of reflections	7690
<I/σ(I)>	15.064		4.527
Completeness [%]	97.4	99.6	82.5
Redundancy	12.7	15.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein Solution (10 MG/ML protein, 0.050 M sodium chloride, 0.0031 M sodium azide, 0.0003 M TCEP, 0.005 M Bis Tris pH 7.0) mixed in a 1:1 ratio with the Well Solution (0.90 M lithium sulfate, 0.10 M sodium succinate pH 4), Cryoprotected with: well solution supplemented with up to 20% glycerol, vapor diffusion, hanging drop, temperature 293K