2O4X

Crystal structure of human P100 tudor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-16
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	49.931, 93.413, 95.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.000 - 2.000
R-factor	0.23427
Rwork	0.233
R-free	0.24919
Structure solution method	Pt-SAS
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.240	2.050
High resolution limit [Å]	2.000	2.000
Rmerge		0.400
Number of reflections	29102

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	289	2.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (10.0mg/ml), RESERVOIR SOLUTION CONTAING 20% PEG 8000, 100.0mM PHOSPHATE, 0.1M HEPES pH 4.2 , VAPOR DIFFUSION, HANGING DROP, temperature 289K