2O4J
Crystal Structure of Rat Vitamin D Receptor Ligand Binding Domain Complexed with VitIII 17-20Z and the NR2 Box of DRIP 205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | BRUKER PROTEUM R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.500, 42.400, 41.900 |
| Unit cell angles | 90.00, 96.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.740 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1rjk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.560 |
| Data reduction software | SAINT |
| Data scaling software | LSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 1.850 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 27577 | |
| <I/σ(I)> | 19.4 | 2.86 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6.1 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macroseeding in batch | 7 | 295 | PEG 4000, MOPS, Ammonium Citrate, Isopropanol, pH 7.0, macroseeding in batch, temperature 295K |
