2O4C
Crystal Structure of D-Erythronate-4-phosphate Dehydrogenase Complexed with NAD
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97930, 0.97946, 0.95000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.914, 101.630, 142.994 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.770 - 2.300 |
R-factor | 0.229 |
Rwork | 0.225 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.173 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.049 | 0.442 |
Number of reflections | 63477 | |
<I/σ(I)> | 14.6 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 297 | 0.7M ammonium dihydrogen phosphate, 0.4M ammonium tartrate, 0.1M sodium citrate (pH 5.6), 10mM cupric chloride, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 297 | 0.7M ammonium dihydrogen phosphate, 0.4M ammonium tartrate, 0.1M sodium citrate (pH 5.6), 10mM cupric chloride, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 297 | 0.7M ammonium dihydrogen phosphate, 0.4M ammonium tartrate, 0.1M sodium citrate (pH 5.6), 10mM cupric chloride, VAPOR DIFFUSION, HANGING DROP, temperature 297K |