2O3V
Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site complexed with paromamine derivative NB33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.620, 41.220, 56.910 |
| Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
| Resolution | 27.730 - 2.800 |
| Rwork | 0.232 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.730 | 27.730 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.010 | 2.800 |
| Rmerge | 0.060 | 0.037 | 0.362 |
| Total number of observations | 1000 | 1144 | |
| Number of reflections | 3225 | ||
| <I/σ(I)> | 11 | 26.4 | 2.7 |
| Completeness [%] | 99.3 | 93.6 | 100 |
| Redundancy | 3.46 | 2.97 | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 300 | Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, pH 6.5, VAPOR DIFFUSION, temperature 300K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Cacodylate | ||
| 2 | 1 | 1 | Potassium chloride | ||
| 3 | 1 | 1 | 2-methyl-2,4-pentanediol | ||
| 4 | 1 | 1 | hexammine cobalt chloride | ||
| 5 | 1 | 2 | Potassium chloride | ||
| 6 | 1 | 2 | 2-methyl-2,4-pentanediol | ||
| 7 | 1 | 2 | hexammine cobalt chloride |
