2O3O
Crystal Structure of the sensor histidine kinase regulator YycI from Bacillus subtitlis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Wavelength(s) | 0.94645, 0.97969, 0.97955 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.792, 161.922, 180.156 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 90.000 - 2.890 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.169 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.970 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.084 | 0.334 |
| Number of reflections | 71733 | |
| <I/σ(I)> | 12.4 | 1.4 |
| Completeness [%] | 92.7 | 53.9 |
| Redundancy | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2 | 293 | 18% PEG1500, 100 MM CITRATE BUFFER, 200 mM lithium sulfate, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 3.20 |
