2O3G
Structural Genomics, the crystal structure of a conserved putative domain from Neisseria meningitidis MC58
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97907, 0.97928 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 129.828, 129.828, 129.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.270 - 2.550 |
| R-factor | 0.24263 |
| Rwork | 0.242 |
| R-free | 0.26046 |
| Structure solution method | MAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.805 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.270 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.055 | 0.484 |
| Number of reflections | 6316 | |
| <I/σ(I)> | 46.98 | 4.65 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 10.2 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.4M Sodium malonate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
