2O3A
Crystal structure of a protein AF_0751 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.294, 55.890, 108.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.035 - 2.200 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.490 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.109 | 0.504 |
| Total number of observations | 19675 | |
| Number of reflections | 16685 | |
| <I/σ(I)> | 18 | 3.7 |
| Completeness [%] | 97.7 | 91.3 |
| Redundancy | 12.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 100mM Hepes pH 7.5, 30% MPD, 200mM tri-sodium citrate dihydrate, Vapor diffusion, temperature 294K |
