2O35
Protein of Unknown Function (DUF1244) from Sinorhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.994, 80.994, 64.708 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.070 - 2.120 |
| R-factor | 0.21407 |
| Rwork | 0.209 |
| R-free | 0.26288 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.556 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.070 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.088 | 0.554 |
| Number of reflections | 13992 | |
| <I/σ(I)> | 6.6 | 1.92 |
| Completeness [%] | 98.4 | 95.7 |
| Redundancy | 16.3 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.4 M Sodium citrate, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
