2O30
Nuclear movement protein from E. cuniculi GB-M1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2006-10-26 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9790156 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.980, 44.171, 67.721 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 67.730 - 1.660 |
| R-factor | 0.21686 |
| Rwork | 0.215 |
| R-free | 0.25645 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.119 | |
| Number of reflections | 20035 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 91.8 | 56.6 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25% PEG3350, 0.2M CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
