2O2E
Mycobacterium tuberculosis tryptophan synthase beta subunit dimer (apoform)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.984, 56.461, 120.545 |
| Unit cell angles | 90.00, 117.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.200 |
| R-factor | 0.21434 |
| Rwork | 0.211 |
| R-free | 0.28072 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wsy |
| RMSD bond length | 0.041 |
| RMSD bond angle | 2.933 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.220 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.074 | 0.582 |
| Number of reflections | 38769 | |
| <I/σ(I)> | 13.5 | 1.76 |
| Completeness [%] | 98.7 | 81.9 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.1M MES, 0.1 K/Na Tartrate, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
