2O29
Spectroscopic and Structural Study of the Heterotropic Linkage between Halide and Proton Ion Binding to Gfp Proteins: E2(GFP)-BR Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.144, 62.852, 69.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.150 - 1.800 |
| R-factor | 0.17909 |
| Rwork | 0.178 |
| R-free | 0.20835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h6v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.523 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.684 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.098 | 0.377 |
| Number of reflections | 20873 | |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 97.7 | 95.2 |
| Redundancy | 5.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 14% (W/V) PEG 3350, 100 MM NH4 ACETATE, 0.2 M NH4Br, PH 5.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 100K |
