2O1Z
Plasmodium vivax Ribonucleotide Reductase Subunit R2 (Pv086155)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.151, 79.978, 103.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.320 - 2.400 |
| R-factor | 0.22055 |
| Rwork | 0.218 |
| R-free | 0.28535 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hon |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (V. 1.3.2) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.112 | 0.456 |
| Number of reflections | 25506 | |
| <I/σ(I)> | 7.9 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 30% PEG 2K MME, 0.15M Potasium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
