2O1W
Structure of N-terminal plus middle domains (N+M) of GRP94
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00520 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.330, 137.500, 133.150 |
| Unit cell angles | 90.00, 124.10, 90.00 |
Refinement procedure
| Resolution | 47.300 - 3.400 |
| R-factor | 0.31475 |
| Rwork | 0.314 |
| R-free | 0.33194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YT1 and homology model based upon 1HK7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 42419 | |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 97.6 | 98.9 |
| Redundancy | 3.5 | 3.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 291 | 0.1M morpholinoethanesulfonic acid (MES), pH 6.0, 40% v/v PEG400, 100mM MgCl2, Microbatch, temperature 291K |
