2O1L
Structure of a complex of C-terminal lobe of bovine lactoferrin with disaccharide at 1.97 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-22 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.609, 50.017, 65.395 |
| Unit cell angles | 90.00, 107.11, 90.00 |
Refinement procedure
| Resolution | 62.020 - 1.970 |
| R-factor | 0.21501 |
| Rwork | 0.214 |
| R-free | 0.23054 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2B6D |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 22864 | |
| Completeness [%] | 90.9 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | HEPES, PEG 550 monomethyl ether, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
