2O17
Pectate lyase bound to hexasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.254, 69.345, 59.826 |
| Unit cell angles | 90.00, 113.69, 90.00 |
Refinement procedure
| Resolution | 45.880 - 2.300 |
| R-factor | 0.14691 |
| Rwork | 0.143 |
| R-free | 0.22315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.246 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.340 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.097 | 0.220 |
| Number of reflections | 15712 | |
| <I/σ(I)> | 11 | 4.2 |
| Completeness [%] | 92.1 | 90.3 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M Sodium acetate pH 4.6, 0.2M Ammonium acetate, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
