2O16
Crystal structure of a putative acetoin utilization protein (AcuB) from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.677, 58.006, 53.561 |
| Unit cell angles | 90.00, 108.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xkf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.481 |
| Number of reflections | 22430 | |
| <I/σ(I)> | 23.8 | 2.8 |
| Completeness [%] | 98.3 | 84.8 |
| Redundancy | 7.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 100mM Tris pH 8.5, 2M ammonium phosphate, VAPOR DIFFUSION, temperature 294K |
