2O14
X-Ray Crystal Structure of Protein YXIM_BACsu from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR595
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-05 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.97908, 0.97932, 0.96797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.425, 59.774, 92.388 |
| Unit cell angles | 90.00, 100.34, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.253 |
| Number of reflections | 47440 | |
| <I/σ(I)> | 13.7 | 3.9 |
| Completeness [%] | 91.5 | 72 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MnSo4.H2O 0.1M, MOPS 0.1M, PEG8000 20% (w/v), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
