2O12
Mycobacterium tuberculosis Chorismate synthase in complex with FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 133.445, 133.445, 158.619 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.740 - 1.720 |
| R-factor | 0.1657 |
| Rwork | 0.165 |
| R-free | 0.18783 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.317 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.740 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.086 | 0.932 |
| Number of reflections | 88273 | |
| <I/σ(I)> | 20.6 | 2.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 4.8 M NH4-Acetate, 0.1 M Na-Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
