2O11
Mycobacterium tuberculosis Chorismate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 133.586, 133.586, 160.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.710 - 1.650 |
| R-factor | 0.18959 |
| Rwork | 0.189 |
| R-free | 0.20796 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.511 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.670 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.075 | 0.448 |
| Number of reflections | 100281 | |
| <I/σ(I)> | 20.5 | 2.8 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 4.8 M NH4-Acetate, 0.1 M Na-Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
