2O0T
The three dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-25 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.780, 106.880, 121.930 |
| Unit cell angles | 90.00, 104.99, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.330 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hkv |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.788 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.370 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.077 | 0.623 |
| Number of reflections | 80203 | |
| <I/σ(I)> | 17.8 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 20-23%(w/v) polyethylene glycol monomethylether 5000, 0.1 M MES, 60 mM ammonium sulfate, pH 6.1-6.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
