2O0Q
X-ray Crystal Structure of Protein CC0527 from Caulobacter crescentus. Northeast Structural Genomics Consortium Target CcR55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 85.143, 104.326, 46.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.980 - 1.800 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o0p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.369 |
| Number of reflections | 31132 | |
| <I/σ(I)> | 11.4 | 9.4 |
| Completeness [%] | 98.4 | 88.3 |
| Redundancy | 7.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2M Sodium acetate, 0.1M Sodium Citrate, PGE 4000 , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
