2O0P
X-ray Crystal Structure of Protein CC0527 (V27M / L66M double mutant) from Caulobacter crescentus. Northeast Structural Genomics Consortium Target CcR55.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X3A |
Synchrotron site | NSLS |
Beamline | X3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 84.864, 103.773, 46.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.850 - 1.900 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.24500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.085 | 0.394 |
Number of reflections | 31207 | |
<I/σ(I)> | 10.4 | 9.04 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.7 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2M Sodium acetate, 0.1M Sodium Citrate, PGE 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2M Sodium acetate, 0.1M Sodium Citrate, PGE 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |