2O0M
The crystal structure of the putative SorC family transcriptional regulator from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.398, 41.660, 64.267 |
| Unit cell angles | 90.00, 118.48, 90.00 |
Refinement procedure
| Resolution | 56.520 - 1.600 |
| R-factor | 0.1659 |
| Rwork | 0.164 |
| R-free | 0.20089 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.520 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.096 | 0.291 |
| Number of reflections | 33635 | |
| <I/σ(I)> | 15.93 | 2.46 |
| Completeness [%] | 0.2 | 93.48 |
| Redundancy | 4.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M Ammonium Sulfate, 0.1M Tris, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
