2NZX

Crystal Structure of alpha1,3-Fucosyltransferase with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	104.154, 136.249, 96.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.192
Rwork	0.190
R-free	0.23700
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.020
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.047	0.603
Number of reflections	104965
<I/σ(I)>	42.7	3.4
Completeness [%]	96.5	91.4
Redundancy	5.9	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	4-5% PEG 3350, 0.2-0.4M ammonium phosphate, 0.1M Bis-Tris , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K