2NZW
Crystal Structure of alpha1,3-Fucosyltransferase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000, 0.9798, 0.9645, 0.9799 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.545, 136.196, 96.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.24200 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.058 | 0.869 |
Number of reflections | 107364 | |
<I/σ(I)> | 35.7 | 2.5 |
Completeness [%] | 98.6 | 94.1 |
Redundancy | 6.5 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 4-5% PEG 3350, 0.2-0.4M ammonium phosphate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |