2NZL
Crystal structure of human hydroxyacid oxidase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 4 |
| Unit cell lengths | 97.294, 97.294, 79.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.060 - 1.350 |
| R-factor | 0.126 |
| Rwork | 0.126 |
| R-free | 0.15500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1GOX 1ltd 1tb3 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 0.033 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.060 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.092 | 0.439 |
| Number of reflections | 81283 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.1M MMT, 30.0% PEG 1K, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.00 |
