2NZH
Crystal structure of a secretion chaperone CsaA from Bacillus subtilis in the space group P 4 21 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 102 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.146, 109.146, 37.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.550 - 1.900 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.606 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.570 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.286 |
| Total number of observations | 1383 | |
| Number of reflections | 17190 | |
| <I/σ(I)> | 31.9 | 7.2 |
| Completeness [%] | 93.3 | 87 |
| Redundancy | 11.11 | 9.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 30% PEG8000, 0.2M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
