2NZ8
N-terminal DHPH cassette of Trio in complex with nucleotide-free Rac1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0712 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.492, 108.558, 53.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.410 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | residues 1231-1390 of PDB entry 1NTY and residues 1-177 of PDB entry 1FOE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39416 | |
| <I/σ(I)> | 15.6 | 3.53 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 100 mM sodium cacodylate pH 5.5 to 6.5, 14 to 18% (w/v) PEG 8000, and 300-500 mM calcium acetate, pH 6.0, VAPOR DIFFUSION, temperature 291K |
