2NZ7
Crystal Structure Analysis of Caspase-recruitment Domain (CARD) of Nod1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.091, 79.091, 80.863 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.530 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26600 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.672 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE (2.11) |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.519 | |
| High resolution limit [Å] | 1.823 | 1.823 |
| Rmerge | 0.076 | 0.516 |
| Number of reflections | 26612 | |
| <I/σ(I)> | 26.4 | 4.6 |
| Redundancy | 10.4 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20 % PEG 6000, 0.067 M MES (pH 6.0), 0.1 M diammonium hydrogen citrate, 0.09 M NaI, 0.001 M DTT, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
