2NZ5
Structure and Function Studies of Cytochrome P450 158A1 from Streptomyces coelicolor A3(2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.267, 44.859, 105.052 |
Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.350 |
R-factor | 0.249 |
Rwork | 0.235 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dkk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.420 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.085 | 0.264 |
Number of reflections | 50519 | |
<I/σ(I)> | 17.2 | 4.8 |
Completeness [%] | 97.0 | 97 |
Redundancy | 3.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | pH 6.5-8.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |