2NZ1

Viral Chemokine Binding Protein M3 From Murine Gammaherpesvirus68 In Complex With The CC-Chemokine CCL2/MCP-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	173
Detector technology	CCD
Collection date	2001-06-22
Detector	ADSC QUANTUM 315
Spacegroup name	P 31 2 1
Unit cell lengths	99.240, 99.240, 243.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.750 - 2.500
R-factor	0.231
Rwork	0.231
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB CODE 1ML0
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.610
High resolution limit [Å]	2.500	2.500
Number of reflections	45669
<I/σ(I)>	11.7	4.1
Completeness [%]	93.4	87.3
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.1	293	12% PEG 4000, 100 mM sodium acetate, 200 mM magnesium chloride, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K