2NYU
Crystal Structure of Human FtsJ homolog 2 (E.coli) protein in complex with S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 |
| Unit cell lengths | 102.022, 102.022, 37.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.760 |
| R-factor | 0.22753 |
| Rwork | 0.226 |
| R-free | 0.28113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eiz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.473 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.048 | 0.629 |
| Number of reflections | 37711 | |
| <I/σ(I)> | 16.2 | 1.65 |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 6.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 20% PEG 3350, 0.2M Potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
