2NYN
Crystal structure of phenylalanine ammonia-lyase from Anabaena variabilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.193, 88.515, 90.058 |
| Unit cell angles | 103.52, 97.82, 116.24 |
Refinement procedure
| Resolution | 100.000 - 1.900 |
| Rwork | 0.223 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w27 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.289 |
| Number of reflections | 159839 | |
| <I/σ(I)> | 7.94 | 3.2 |
| Completeness [%] | 94.8 | 88.4 |
| Redundancy | 2.15 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M MOPSO, 7% (w/v) polyethylene glycol 8000, 0.2 M magnesium chloride, 10 mM cinnamic acid, 2 mM dithiothreitol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
