2NYK
Crystal structure of m157 from mouse cytomegalovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.028, 43.805, 62.321 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.21507 |
| Rwork | 0.214 |
| R-free | 0.24028 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.260 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 | 0.359 |
| Number of reflections | 18770 | |
| <I/σ(I)> | 15.2 | 3.6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 100mM MES pH 6.0, 200mM ammonium acetate, 35% PEG400, VAPOR DIFFUSION, temperature 293K |
