2NY6
HIV-1 gp120 Envelope Glycoprotein (M95W, W96C, I109C, T123C, T257S, V275C,S334A, S375W, Q428C, G431C) Complexed with CD4 and Antibody 17b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 72.310, 87.018, 196.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.370 - 2.800 |
| R-factor | 0.1959 |
| Rwork | 0.192 |
| R-free | 0.27577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.151 | 0.070 | 0.581 |
| Number of reflections | 28525 | ||
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 92.5 | 96.7 | 65 |
| Redundancy | 3.4 | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 8.3% PEG 8000, 7.4% MPD, 100 mM Na Citrate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
