2NXZ
HIV-1 gp120 Envelope Glycoprotein (T257S, S334A, S375W) Complexed with CD4 and Antibody 17b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-13 |
| Detector | MAR CCD 225 mm |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 71.915, 88.126, 197.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.380 - 2.040 |
| R-factor | 0.20821 |
| Rwork | 0.206 |
| R-free | 0.24425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9c |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.997 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
| High resolution limit [Å] | 2.040 | 4.420 | 2.040 |
| Rmerge | 0.114 | 0.063 | 0.505 |
| Number of reflections | 74195 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 92.2 | 97.9 | 68.2 |
| Redundancy | 4.5 | 5.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 7.5% PEG 4000, 8.5% MPD, 100 mM Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
