2NXY
HIV-1 gp120 Envelope Glycoprotein(S334A) Complexed with CD4 and Antibody 17b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-04 |
| Detector | MAR CCD 225 mm |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 71.346, 88.095, 195.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.010 - 2.000 |
| R-factor | 0.18308 |
| Rwork | 0.181 |
| R-free | 0.23123 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.916 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.160 | 0.074 | 0.735 |
| Number of reflections | 82862 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 98.1 | 99.8 | 91.3 |
| Redundancy | 5.6 | 7.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 7.0 % PEG 8000, 7.5 % 1,6-Hexanediol, 100 mM Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
