2NXJ
T.thermophilus ribosomal protein L11 methyltransferase (PrmA) in space group P 21 21 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 124.195, 153.622, 33.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.21139 |
| Rwork | 0.208 |
| R-free | 0.27624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.731 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.417 |
| Number of reflections | 28554 | |
| <I/σ(I)> | 15.4 | 2.2 |
| Completeness [%] | 96.8 | 85.8 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.7 M Ammonium sulfate, 85 mM HEPES-Na, pH 7.5, 1.7% w/v PEG400, 15% v/v glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
