2NWR
Crystal structure of C11N mutant of KDO8P Synthase in complex with PEP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.406, 84.406, 159.428 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.630 - 1.500 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.087 |
| Number of reflections | 103074 |
| <I/σ(I)> | 3.9 |
| Completeness [%] | 97.4 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
