2NWI
Crystal structure of protein AF1396 from Archaeoglobus fulgidus, Pfam DUF98
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-11-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.131, 128.647, 129.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.413 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.881 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.165 | 0.737 |
| Total number of observations | 55734 | |
| Number of reflections | 57535 | |
| <I/σ(I)> | 13.5 | 2.6 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 8.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | 100mM Bis-Tris, 30% PEG MME 550, 50mM calcium chloride, pH 6.5, VAPOR DIFFUSION, temperature 294K |
