2NWF
Soluble domain of Rieske Iron Sulfur Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 70.637, 70.637, 54.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.330 - 1.100 |
| R-factor | 0.12478 |
| Rwork | 0.124 |
| R-free | 0.13811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nuk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.719 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.330 | 1.150 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.050 | 0.130 |
| Number of reflections | 54430 | |
| <I/σ(I)> | 16.7 | 12 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 276 | 1.5 M ammonium sulfate, 20% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 276K |
