2NW2
Crystal structure of ELS4 TCR at 1.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Collection date | 2004-11-07 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.564, 84.163, 59.270 |
| Unit cell angles | 90.00, 107.34, 90.00 |
Refinement procedure
| Resolution | 30.560 - 1.400 |
| R-factor | 0.19405 |
| Rwork | 0.192 |
| R-free | 0.22353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.476 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.350 |
| Number of reflections | 78865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 0.2M Ca(CH3COO)2, 0.1M TRIS (pH7.2), 18% PEG 3350, vapor diffusion, hanging drop, temperature 277K |
