2NW0
Crystal structure of a lysin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.055, 65.773, 66.747 |
| Unit cell angles | 90.00, 85.08, 90.00 |
Refinement procedure
| Resolution | 66.519 - 1.600 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jfx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.520 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.071 | 0.498 |
| Total number of observations | 16065 | |
| Number of reflections | 47219 | |
| <I/σ(I)> | 7.1 | 1.5 |
| Completeness [%] | 92.3 | 54.3 |
| Redundancy | 6.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | 32% PEG 4000, 0.1M sodium acetate, 0.2M ammonium acetate, 0.009M Copper (2) chloride, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
