2NVZ
RNA Polymerase II elongation complex with UTP, updated 11/2006
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.647, 222.338, 194.316 |
Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
Resolution | 40.000 - 4.300 |
R-factor | 0.27605 |
Rwork | 0.270 |
R-free | 0.33165 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sfo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.364 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.300 |
High resolution limit [Å] | 4.200 | 4.200 |
Number of reflections | 50043 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 95.8 | 93.2 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 50mM dioxane, 10mM DTT, 9-12% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2HPO4 | ||
2 | 1 | 1 | NaH2PO4 | ||
3 | 1 | 1 | dioxane | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 1 | PEG6000 | ||
6 | 1 | 2 | (NH4)2HPO4 | ||
7 | 1 | 2 | NaH2PO4 | ||
8 | 1 | 2 | PEG6000 |