2NVW

Crystal sctucture of transcriptional regulator Gal80p from kluyveromymes lactis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-06
Detector	SBC-3
Wavelength(s)	0.97924, 0.97907, 0.96411
Spacegroup name	P 21 21 2
Unit cell lengths	112.200, 137.100, 72.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.197
Rwork	0.195
R-free	0.24500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	2.590
Data reduction software	HKL-2000
Data scaling software	SAINT
Phasing software	SOLVE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	65527
<I/σ(I)>	16	2
Completeness [%]	97.7	94.2
Redundancy	6.5	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.25	277	20% 2,4-MPD, 0.2M NaCl, 0.1M MES, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K