2NVT
RNA Polymerase II Elongation Complex in 150 mM Mg+2 with GMPCPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.872, 222.824, 195.802 |
| Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.360 |
| R-factor | 0.23223 |
| Rwork | 0.230 |
| R-free | 0.28289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfo |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.822 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.500 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Number of reflections | 95301 | |
| <I/σ(I)> | 8 | 1.4 |
| Completeness [%] | 97.1 | 94.8 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200mM NH4OAc, 150mM Mg(OAc)2, 50mM Hepes, pH 7.0, 5% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NH4OAc | ||
| 2 | 1 | 1 | Mg(OAc)2 | ||
| 3 | 1 | 1 | Hepes | ||
| 4 | 1 | 1 | PEG6000 | ||
| 5 | 1 | 2 | NH4OAc | ||
| 6 | 1 | 2 | Mg(OAc)2 | ||
| 7 | 1 | 2 | Hepes | ||
| 8 | 1 | 2 | PEG6000 |
